The Radish GenomeThe Radish Genome

This book summarizes the latest information and the status quo of radish genome studies to stimulate innovations and improvements in breeding techniques and to promote further advances in the field.

Author: Takeshi Nishio

Publisher: Springer

ISBN: 9783319592534


Page: 220

View: 918

This book summarizes the latest information and the status quo of radish genome studies to stimulate innovations and improvements in breeding techniques and to promote further advances in the field. Radish (Raphanus sativus) is a member of the Brassicaceae family and is cultivated worldwide. Its varieties have been diversified in terms of size, shape, and the color of their roots and bio-components. Thanks to the development of high-throughput molecular techniques using next generation sequencers, complete genomes of cultivated and wild radish plants have been sequenced and published with annotations of predicted genes and single nucleotide polymorphism (SNP) information between radish cultivars and accessions. These, together with the construction of a high-density genetic map of radish and profiling of expression sequences in radish organs, have accelerated genetic studies, such as the identification of genes or loci associated with root development, pungent components, and plant disease resistance. Providing an overview of these advances, this book is a valuable resource for scientists involved in plant genetic research and crop breeding.

Genome Mapping of Radish Raphanus Sativus L Using PCR Based MarkersGenome Mapping of Radish Raphanus Sativus L Using PCR Based Markers

This book was prepared to describe the scientific principles of the construction of genetic maps using an intera-specific F2 population and two PCR-based markers RAPDs and AFLPs.

Author: Magdi Mousa

Publisher: LAP Lambert Academic Publishing

ISBN: 3659153087


Page: 76

View: 740

This book was prepared to describe the scientific principles of the construction of genetic maps using an intera-specific F2 population and two PCR-based markers RAPDs and AFLPs. The book also describes the utilization of the construction of genetic maps in plant breeding programs, including gene(s)/QTL(s) mapping of desired traits, gene(s) isolation and gene(s) transfer. The mapped gene(s) can be used successfully in marker assisted selection (MAS) programs. This book will be a value added scientific resource for the MSc and PhD students who are interested to do their thesis's and dissertations in the field of plant genome, gen(s) and QTL(s) mapping. Simultaneously, the presented work well be a helpful lab manual for the young scientists and Post-Doc researchers due to the powerful information, protocols', techniques and detailed statistical methodology were included in the book chapters.

Genetics and Genomics of the BrassicaceaeGenetics and Genomics of the Brassicaceae

These are reviewed, including germplasm and genomic library resources, and resources for reverse genetics, metabolomics, bioinformatics and transformation.

Author: Renate Schmidt

Publisher: Springer Science & Business Media

ISBN: 9781441971180


Page: 680

View: 803

The Genetics and Genomics of the Brassicaceae provides a review of this important family (commonly termed the mustard family, or Cruciferae). The family contains several cultivated species, including radish, rocket, watercress, wasabi and horseradish, in addition to the vegetable and oil crops of the Brassica genus. There are numerous further species with great potential for exploitation in 21st century agriculture, particularly as sources of bioactive chemicals. These opportunities are reviewed, in the context of the Brassicaceae in agriculture. More detailed descriptions are provided of the genetics of the cultivated Brassica crops, including both the species producing most of the brassica vegetable crops (B. rapa and B. oleracea) and the principal species producing oilseed crops (B. napus and B. juncea). The Brassicaceae also include important “model” plant species. Most prominent is Arabidopsis thaliana, the first plant species to have its genome sequenced. Natural genetic variation is reviewed for A. thaliana, as are the genetics of the closely related A. lyrata and of the genus Capsella. Self incompatibility is widespread in the Brassicaceae, and this subject is reviewed. Interest arising from both the commercial value of crop species of the Brassicaceae and the importance of Arabidopsis thaliana as a model species, has led to the development of numerous resources to support research. These are reviewed, including germplasm and genomic library resources, and resources for reverse genetics, metabolomics, bioinformatics and transformation. Molecular studies of the genomes of species of the Brassicaceae revealed extensive genome duplication, indicative of multiple polyploidy events during evolution. In some species, such as Brassica napus, there is evidence of multiple rounds of polyploidy during its relatively recent evolution, thus the Brassicaceae represent an excellent model system for the study of the impacts of polyploidy and the subsequent process of diploidisation, whereby the genome stabilises. Sequence-level characterization of the genomes of Arabidopsis thaliana and Brassica rapa are presented, along with summaries of comparative studies conducted at both linkage map and sequence level, and analysis of the structural and functional evolution of resynthesised polyploids, along with a description of the phylogeny and karyotype evolution of the Brassicaceae. Finally, some perspectives of the editors are presented. These focus upon the Brassicaceae species as models for studying genome evolution following polyploidy, the impact of advances in genome sequencing technology, prospects for future transcriptome analysis and upcoming model systems.

Fine Mapping and Functional Analysis of the Radish Rfo Nuclear Restorer LocusFine Mapping and Functional Analysis of the Radish Rfo Nuclear Restorer Locus

"Cytoplasmic male sterility (CMS) is a widespread, maternally inherited trait that results in an inability of plants to produce functional pollen.

Author: Richard Burns Wargachuk


ISBN: OCLC:61740670


Page: 100

View: 419

"Cytoplasmic male sterility (CMS) is a widespread, maternally inherited trait that results in an inability of plants to produce functional pollen. The Ogura CMS system originated in radish, but has since been transferred to, and confers male sterility on, plants in the related genus Brassica . A gene which restores male fertility is needed for the Ogura CMS system to be exploited commercially for hybrid seed production in oilseed species such as Brassica napus. The restorer gene Rfo is a dominant radish nuclear gene that restores the male fertility to plants with Ogura cytoplasm. This gene has been transferred into Brassica napus through intergeneric crosses; however the introgressed segment of radish DNA contains an unknown number of genes, some of which confer undesirable traits, such as an elevated content of seed glucosinolates, antinutritive compounds that render the seed meal unusable as animal feed. A fine scale linkage map of the region in radish containing Rfo was constructed, and a map-based cloning approach relying on synteny between radish and Arabidopsis was used to clone Rfo. A radish gene encoding a 687 amino acid protein with a predicted mitochondrial targeting presequence was found to confer male fertility upon transformation into Ogura CMS B. napus . This gene, codes for a pentatricopeptide repeat (PPR)-containing protein with multiple, in this case 16, PPR domains. Two similar genes that do not appear to function as Rfo flank this gene. A transcript representing a non-functional allele (rfo) was detected in sterile radish plants. Comparison of the Rfo region with the syntenic Arabidopsis region indicates that a PPR gene is not present at the Rfo-equivalent site in Arabidopsis , although a smaller and related PPR gene is found about 40 kb from this site." --

Achieving Nutritional Security and Food Safety Through Genomics Based Breeding of CropsAchieving Nutritional Security and Food Safety Through Genomics Based Breeding of Crops

bThe physical position of genes in the candidate region of the reference genome “XYB36-2”. ... Radish genesa Gene position on R02b AT IDc E-value AT GO annotationd Rsa10008436 Rsa10008435 Rsa10008434 Rsa10008433 Rsa10008432 Rsa10008431 ...

Author: Reyazul Rouf Mir

Publisher: Frontiers Media SA

ISBN: 9782889666119



View: 495

Advances in Botanical ResearchAdvances in Botanical Research

Radish genes linked to Rfo restorer gene were detected in the restored rapeseed genome by application of ACGMs (Brunel et al., 1999), indicating several breaks of colinearity between radish and Arabidopsis genomes.

Author: Surinder Kumar Gupta

Publisher: Academic Press

ISBN: 0080548288


Page: 584

View: 303

Rapeseed is an important oilseed crop belonging to Crucifereae family and grown in subtropical to temperate climate. Recent discoveries have caused the scientific community to respond positively by directing a greater amount of research towards increasing production and improving the quality of rapeseed oil. Today, the annual worldwide production is approximately 7.5 million tons on 4 million acres. Canola ranks 5th in the production of world's oilseed crops following soybean, sunflower, groundnut and cottonseed. Rapeseed Breeding fully explains the miraculous discoveries about the genetic material which have contributed to the growth of this important crop. With contributions from world-renowned researchers from North America, Europe, Asia, and Australia, this book provides the first scientific reference for scientists interested in the further exploitation of this important crop. * Presents history, origin and evolution, breeding methods, practical applications of DNA markers, fingerprinting of cultivars, and conservation of rapeseed germplasm * Includes detail of different breeding purposes including breeding for improved oil and meal quality, breeding for winter hardiness, breeding for herbicides, and breeding for hybrid rape. * Provides analysis of ecology, usage, degeneration and application

Genetics Genomics and Breeding of Vegetable BrassicasGenetics Genomics and Breeding of Vegetable Brassicas

By applying emerging genomic technologies, we may greatly increase our understanding of the Brassica biology and breeding efficiency. This volume contains 11

Author: Jan Sadowski

Publisher: CRC Press

ISBN: 9781439866047


Page: 450

View: 337

Due to their diversity, vegetable Brassicas are of great economic import and offer unique opportunities to enrich our knowledge about plant growth, development, and rapid phenotypic evolution. By applying emerging genomic technologies, we may greatly increase our understanding of the Brassica biology and breeding efficiency. This volume contains 11 chapters contributed by 34 specialists with extensive experience in genetics, molecular breeding, and genomics of vegetable Brassicas. Recent achievements and new technologies presented in this book will provide support to further research the genetics and genomics of vegetable Brassica crops and facilitate their genetic improvement.

Evolution of Gene Regulatory Networks in Plant DevelopmentEvolution of Gene Regulatory Networks in Plant Development

classified into two broad groups, type I and type II genes, which were generated by single gene duplication ... Fortunately, the completion of the radish genome sequencing makes it possible to analyze MADS-box genes (Mitsui et al., ...

Author: Federico Valverde

Publisher: Frontiers Media SA

ISBN: 9782889454075



View: 749

During their life cycle plants undergo a wide variety of morphological and developmental changes. Impinging these developmental processes there is a layer of gene, protein and metabolic networks that are responsible for the initiation of the correct developmental transitions at the right time of the year to ensure plant life success. New omic technologies are allowing the acquisition of massive amount of data to develop holistic and integrative analysis to understand complex processes. Among them, Microarray, Next-generation Sequencing (NGS) and Proteomics are providing enormous amount of data from different plant species and developmental stages, thus allowing the analysis of gene networks globally. Besides, the comparison of molecular networks from different species is providing information on their evolutionary history, shedding light on the origin of many key genes/proteins. Moreover, developmental processes are not only genetically programed but are also affected by internal and external signals. Metabolism, light, hormone action, temperature, biotic and abiotic stresses, etc. have a deep effect on developmental programs. The interface and interplay between these internal and external circuits with developmental programs can be unraveled through the integration of systematic experimentation with the computational analysis of the generated omics data (Molecular Systems Biology). This Research Topic intends to deepen in the different plant developmental pathways and how the corresponding gene networks evolved from a Molecular Systems Biology perspective. Global approaches for photoperiod, circadian clock and hormone regulated processes; pattern formation, phase-transitions, organ development, etc. will provide new insights on how plant complexity was built during evolution. Understanding the interface and interplay between different regulatory networks will also provide fundamental information on plant biology and focus on those traits that may be important for next-generation agriculture.

Mitochondrial Genome EvolutionMitochondrial Genome Evolution

Each restorer locus spans a physical region containing numerous PPRs, with high homology to each other suggesting recent tandem duplication or transposition, and phylogenetically close to Rf-PPR from petunia or radish.

Author: Laurence Maréchal-Drouard

Publisher: Academic Press

ISBN: 9780123942791


Page: 436

View: 813

Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume features reviews on Mitochondrial genome evolution. Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences Features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology This thematic volume features reviews on mitochondrial genome evolution

Accelerated Plant Breeding Volume 2Accelerated Plant Breeding Volume 2

The first reported production of transgenic radish by a method known as 'floral dip' used the commercially important Korean cultivar 'Jin Ju Dae Pyong'. This procedure of gene transfer is currently the only system available for ...

Author: Satbir Singh Gosal

Publisher: Springer Nature

ISBN: 9783030472986


Page: 455

View: 865

Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commercial export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This edited volume summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.


They could identify two radish chromosomes added to the rape genome and confirmed that one of them had a close relation to nematode resistance. Recently, Peterka et al. (2004) confirmed that a “d” chromosome of radish carried a nematode ...

Author: Chittaranjan Kole

Publisher: Springer Science & Business Media

ISBN: 9783540345367


Page: 380

View: 298

Vegetables contains reviews in 12 chapters contributed by 31 authors from 10 countries. The impressive work that has been done on most of these crops is presented in this volume. Genome projects already initiated on vegetable crops, particularly on Solanaceae and Brassicaceae species, may ignite further interest in other vegetables as well.


The Japanese radish cv. Kosena has also been used to transfer CMS to B. napus (Sakai and Ima- mura 1990). Iwabuchi et al. (1999) showed that the CMS-associated gene orf125 from Kosena radish has a sequence homologous to that of the ...

Author: Chittaranjan Kole

Publisher: Springer Science & Business Media

ISBN: 9783540343882


Page: 302

View: 409

Part of the seven-volume series Genome Mapping and Molecular Breeding in Plants, the volume Oilseeds is devoted to oil-producing field crops such as soybeans, oilseed rape, peanuts, sunflowers, Indian mustard, Brassica rapa, black mustard and flax. While the grouping of economic plants is conventionally based on their agricultural purposes, several crops covered in this volume have other uses besides yielding oils. Brassica rapa is also used as a vegetable, the sunflower as an ornamental, and flax as a fibre crop. Black mustard, which is used as a condiment but is genetically close to other Brassica species, is also included here.

Brassicas and Legumes From Genome Structure to BreedingBrassicas and Legumes From Genome Structure to Breeding

3.43.5 Fertility Restorer Gene Molecular markers have been generated for the two endogenous (nap and pol) and one introgressed (ogu from radish) nuclear restorer genes of the cytoplasmic male sterile (CMS) system in B. napus.

Author: Toshiyuki Nagata

Publisher: Springer Science & Business Media

ISBN: 9783662050361


Page: 268

View: 288

Genome sequence studies have become more and more important for plant breeding. Brassicas and Legumes: From Genome Structure to Breeding comprises 16 chapters and presents both an overview and the latest results of this rapidly expanding field. Topics covered include: genome analysis of a flowering plant, Arabidopsis thaliana; the sequence of the Arabidopsis genome as a tool for comparative structural genomics in Brassicaceae; application of molecular markers in Brassica coenospecies; the molecular genetic basis of flowering time variation in Brassica species; quantitative trait loci for clubroot resistance in Brassica oleracea; structural differences of S locus between Brassica oleracea and Brassica rapa; Brassica and legume chromosomes; sequence analysis of the Lotus japonicus genome; introduction of an early flowering accession ‘Miyakojima’ MG-20 to molecular genetics in Lotus japonicus; genetic linkage map of the model legume Lotus japonicus; construction of a high quality genome library of Lotus japonicus; genome analysis of Mesorhizobium loti: a symbiotic partner to Lotus japonicus; molecular linkage map of the model legume Medicago truncatula; genetic mapping of seed and nodule protein markers in diploid alfalfa (Medicago sativa); mapping the chickpea (Cicer arietinum) genome: localization of fungal resistance genes in interspecific crosses.

Plant Protein and Proteome Altlas Integrated Omics Analyses of Plants under Abiotic StressesPlant Protein and Proteome Altlas Integrated Omics Analyses of Plants under Abiotic Stresses

The radish genome and comprehensive gene expression profile of tuberous root formation and development. Sci. Rep. 2015, 5, 10835. [CrossRef] [PubMed] Wang, R.; Mei, Y.; Xu, L.; Zhu, X.; Wang, Y.; Guo, J.; Liu, ...

Author: Tingyun Kuang

Publisher: MDPI

ISBN: 9783039219605


Page: 558

View: 513

Integrative omics of plants in response to stress conditions play more crucial roles in the post-genomic era. High-quality genomic data provide more deeper understanding of how plants to survive under environmental stresses. This book is focused on concluding the recent progress in the Protein and Proteome Atlas in plants under different stresses. It covers various aspects of plant protein ranging from agricultural proteomics, structure and function of proteins, and approaches for protein identification and quantification. A total of 27 papers including two timely reviews have contributed to this Special Issue. In the first part with the topic of “Comparative Proteomics of Different Plants”, six papers were included to describe the phenotypic changes and proteomic analyses of different plants under different conditions. Then, another six papers with the topic of “Proteomics of Plants under Osmotic Stress” were included to describe the recent comparative proteomics analyses of plants under osmotic stress, particularly the drought and salinity stresses in leaves of certain plant species. The other proteomics studies on several energy plants and economic crops were reported to demonstrate the recent omics studies on different plants during their development processes. More stress responsive genes and proteins in these plants were identified. These target genes and proteins are important candidates for further functional validation in economic plants and crops.

The Brassicaceae Agri Horticultural and Environmental PerspectivesThe Brassicaceae Agri Horticultural and Environmental Perspectives

For radish, many researches reported that the genomic information have recently been analyzed. In recent years, the genomic and transcriptome information of radish have been extensively clarified. For instance, two leaf and two root ...

Author: Naser A. Anjum

Publisher: Frontiers Media SA

ISBN: 9782889456451



View: 406

This Frontiers Research Topic "The Brassicaceae- Agri-Horticultural and Environmental Perspectives" is an effort to provide a common platform to agronomists, horticulturists, plant breeders, plant geneticists/molecular biologists, plant physiologists and environmental plant scientists exploring major insights into the role of important members of the plant family Brassicaceae (the mustard family, or Cruciferae) in agri-horticultural and environmental arenas.

Progress in Botany 69Progress in Botany 69

radish genome segment in the Brassica restorer line has made improvement of the line very difficult. Primard-Brisset et al. (2005) successfully used gamma ray irradiation to induce chromosome breakage in order to force non-spontaneous ...

Author: Ulrich Lüttge

Publisher: Springer Science & Business Media

ISBN: 9783540729549


Page: 479

View: 963

With one volume published each year, this series keeps scientists and students current with the latest developments and results in all areas of the plant sciences. This present volume includes insightful reviews covering genetics, cell biology, physiology, comparative morphology, systematics, ecology, and vegetation science.

Recent Advances in Genetics and Breeding of Major Staple Food CropsRecent Advances in Genetics and Breeding of Major Staple Food Crops

Burrows–Wheeler Aligner (BWA) software [26] was then used to assemble and to align the clean reads from each individual against the radish's reference genome. Genomic data (Rs 1.0 chromosome) at the chromosome pseudomolecule level of ...

Author: Joong Hyoun Chin

Publisher: MDPI

ISBN: 9783036509761


Page: 314

View: 949

To meet the global food demand of an increasing population, food production has to be increased by 60% by 2050. The main production constraints, such as climate change, biotic stresses, abiotic stresses, soil nutrition deficiency problems, problematic soils, etc., have to be addressed on an urgent basis. More than 50% of human calories are from three major cereals: rice, wheat, and maize. The harnessing of genetic diversity by novel allele mining assisted by recent advances in biotechnological and bioinformatics tools will enhance the utilization of the hidden treasures in the gene bank. Technological advances in plant breeding will provide some solutions for the biofortification, stress resistance, yield potential, and quality improvement in staple crops. The elucidation of the genetic, physiological, and molecular basis of useful traits and the improvement of the improved donors containing multiple traits are key activities for variety development. High-throughput genotyping systems assisted by bioinformatics and data science provide efficient and easy tools for geneticists and breeders. Recently, new breeding techniques applied in some food crops have become game-changers in the global food crop market. With this background, we invited 18 eminent researchers working on food crops from across the world to contribute their high-quality original research manuscripts. The research studies covered modern food crop genetics and breeding: plant molecular systems focusing to food crops; plant genetic diversity—QTL and gene identification utilizing high-throughput genotyping systems and their validation; new breeding techniques in food crops—targeted mutagenesis, genome editing, etc.; abiotic and biotic stresses—QTL/gene identification and their molecular physiology; plant nutrition, grain quality improvement, and yield enhancement.

Somatic Genome VariationSomatic Genome Variation

Brown G.G., Formanova N., Jin H., Wargachuk R., Dendy C., Patil, P., Laforest M., Zhang J., Cheung W.Y., and Landry B.S. (2003) The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple ...

Author: Xiu-Qing Li

Publisher: John Wiley & Sons

ISBN: 9781118647066


Page: 448

View: 892

Written by an international team of experts, Somatic Genome Variation presents a timely summary of the latest understanding of somatic genome development and variation in plants, animals, and microorganisms. Wide-ranging in coverage, the authors provide an updated view of somatic genomes and genetic theories while also offering interpretations of somatic genome variation. The text provides geneticists, bioinformaticians, biologist, plant scientists, crop scientists, and microbiologists with a valuable overview of this fascinating field of research.

The Plant Family BrassicaceaeThe Plant Family Brassicaceae

A gene of GRAS family, i.e., PeSCL7 from poplar used to improve resistance against drought plus salt in Arabidopsis (Ma et al. 2010). BrLAS a gene from B. ... Currently, from radish genome database 126 WRKY putative genes retrieved.

Author: Mirza Hasanuzzaman

Publisher: Springer Nature

ISBN: 9789811563454


Page: 531

View: 499

This book provides all aspects of the physiology, stress responses and tolerance to abiotic stresses of the Brassicaceae plants. Different plant families have been providing food, fodder, fuel, medicine and other basic needs for the human and animal since the ancient time. Among the plant families, Brassicaceae has special importance for their agri-horticultural importance and multifarious uses apart from the basic needs. Interest understanding the response of Brassicaceae plants toward abiotic stresses is growing considering the economic importance and the special adaptive mechanisms. The knowledge needs to be translated into improved elite lines that can contribute to achieve food security. The physiological and molecular mechanisms acting on Brassicaceae introduced in this book are useful to students and researchers working on biology, physiology, environmental interactions and biotechnology of Brassicaceae plants.

Genetics Genomics and Breeding of Oilseed BrassicasGenetics Genomics and Breeding of Oilseed Brassicas

6.5 Conclusion Physical maps are useful tools for capturing specific regions of the genome and provide a robust mechanism for ... (2003) The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple ...

Author: Dave Edwards

Publisher: CRC Press

ISBN: 9781439883358


Page: 438

View: 205

The book describes the history of Brassica oilseed crops, introduces the Brassica genome, its evolution, diversity, classical genetic studies, and breeding. It also delves into molecular genetic linkage and physical maps, progress with genome sequencing initiatives, mutagenesis approaches for trait improvement, proteomics, metabolomics, and bioinfo