Author: Beatty James 1870-
Publisher: Hardpress Publishing
Unlike some other reproductions of classic texts (1) We have not used OCR(Optical Character Recognition), as this leads to bad quality books with introduced typos. (2) In books where there are images such as portraits, maps, sketches etc We have endeavoured to keep the quality of these images, so they represent accurately the original artefact. Although occasionally there may be certain imperfections with these old texts, we feel they deserve to be made available for future generations to enjoy.
About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work.
Author: James Beatty
Publisher: Forgotten Books
Excerpt from The Method of Enzyme Action In offering this working hypothesis for consideration I do not claim that the central idea is in any respect new. All I can say is that I have not met anywhere with the conception of the essential unity of the action of both hydrolysing and oxidising enzymes, nor an account of the mechanism similar to that given. It is quite possible, of course, that both have been previously suggested. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.
A particularly useful variant on standard HPLC methods of measuring enzyme activity is the so-called “semi-continuous” method of HPLC analysis (Harrison et al. 1989). According to this method, the reaction solution of enzyme and ...
Author: Ross L. Stein
Publisher: John Wiley & Sons
Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the pharmaceutical industry has in this field. Beginning with the most basic principles pertaining to simple, one-substrate enzyme reactions and their inhibitors, and progressing to a thorough treatment of two-substrate enzymes, Kinetics of Enzyme Action: Essential Principles for Drug Hunters provides biochemists, medicinal chemists, and pharmaceutical scientists with numerous case study examples to outline the tools and techniques necessary to perform, understand, and interpret detailed kinetic studies for drug discovery.
The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text.
Author: Alane Beatriz Vermelho
Publisher: Bentham Science Publishers
Methods to Determine Enzymatic Activity is a textbook about industrial enzymes. The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text. The main objective of this textbook is to provide readers information focused on the current analysis methods of enzymatic activity at qualitative and quantitative levels. Each chapter is about one specific enzyme and contains information about its substrate and some biochemical properties. The methodologies are presented as an experimental protocol allowing interested readers to reproduce the experimental methods detailed within the textbook. These protocols contain the principle of the technique, materials, methods, and all steps necessary for the determination of enzyme activity and interpretation of results. Each methodology is illustrated with photos and schemes for a better and clear understanding. This book, therefore, uniquely brings modern analysis techniques of industrial enzymes in a single easy to understand volume. This textbook is suitable for undergraduate enzymology courses and advanced industrial biotechnology and microbiology courses.
In the year 1958 , I devised a method of capturing enzyme activity on film by motion picture microphotography . The description and plates shown in Figure 2.1 are reproduced by permission of the National Enzyme Company . The action ...
Author: Edward Howell
Why is eating food in its natural state, unprocessed and unrefined, so vital to the maintenance of good health? What is lacking in our modern diet that makes us so susceptible to degenerative disease? What natural elements in food may play a key role in unlocking the secrets of life extension? These fascinating questions, and many more, are answered in Enzyme Nutrition. Written by one of America’s pioneering biochemists and nutrition researchers, Dr. Edward Howell, Enzyme Nutrition presents the most vital nutritional discovery since that of vitamins and minerals—food enzymes. Our digestive organs produce some enzymes internally, however food enzymes are necessary for optimal health and must come from uncooked foods such as fresh fruits and vegetables, raw sprouted grains, unpasteurized dairy products, and food enzyme supplements. Enzyme Nutrition represents more than fifty years of research and experimentation by Dr. Howell. He shows us how to conserve our enzymes and maintain internal balance. As the body regains its strength and vigor, its capacity to maintain its normal weight, fight disease, and heal itself is enhanced.
Methods in Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes.
Author: Richard P. Dick
Methods in Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background information, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.
In the face of rapid developments in automated techniques, this book presents an excellent guide to present trends.
Author: D.B. Roodyn
In the face of rapid developments in automated techniques, this book presents an excellent guide to present trends. It provides information on: principles and terminology of enzyme automation; automatic methods illustrated by the Technicon system; semi-automatic methods; interrupted-flow and discrete-sampling systems; single-enzyme analysis; multiple-enzyme analysis (M.E.A.); enzyme characterization; calculation of enzyme activities from instrument readings; generalized systems for enzyme automation; Appendices I. Published automated enzyme assays, II. Terminology used in enzyme automation, III. Apparatus used in enzyme automation, IV. Computer program for generalized enzyme automated system.
The method of Potter  also has relatively low contamination levels of 0.2–0.4%. ... George and Abernethy  have made use of propionylthiocholine as substrate and the detergent benzethonium chloride to stop the enzyme action.
Author: Bryan Ballantyne
Clinical & Experimental Toxicology of Organophosphates and Carbamates considers the histochemical localization of cholinesterase in anticholinesterase poisoning. This book is organized into eight parts encompassing 59 chapters that discuss the pharmacology and toxicology of carbamates, as well as the neurobehavioral toxicology of anticholinesterases. Some of the topics covered in the book are the overview of biological and clinical aspects of organophosphates and carbamates; biochemical determination of cholinesterase activity in fluids and tissues; intermediate syndrome in anticholinesterase neurotoxicity; structure-activity relationships and anticholinesterase activity; and the molecular events in delayed neuropathy. Other parts deal with the cardiac effects of anticholinesterase agents and the ecotoxicological consequences of interactions between avian esterases and organophosphorus compounds. These topics are followed by discussions on the analysis of the cutaneous and respiratory tract absorption of anticholinesterases and the use of organophosphorus compounds as chemical warfare agents. The final parts are devoted to the toxicity of anticholinesterases to aquatic organisms. The book can provide useful information to toxicologists, doctors, students, and researchers.
Examples of this are the detection of trypsin (220.127.116.11 — T; Other Methods, A), esteroprotease (18.104.22.168 — EP, Method 2), and carbonic anhydrase (22.214.171.124 — CA, Method 1). The orthophosphate, liberated by enzymatic activity, ...
Author: Gennady P. Manchenko
Publisher: CRC Press
Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish
al., 1931) formed due to enzyme action. Most of the old methods were based on the titration of the gallic acid liberated by enzyme action (Freudenberg et al., 1927; Nishira, 1961; Haslam and Stangroom, 1966) and hence do not yield ...
Publisher: Academic Press
Praise for the Series "No laboratory scientist, field worker or technical administrator can afford to pass it up." --ASM News "The topics are well supported by an extensive bibliography and provide a rich source of current information." --Biopharm
authors, a further disadvantage is that it cannot be used with crude enzyme preparations of low specific activity, because the contribution of the change in conductivity due to enzyme action may be very small, as compared with that of ...
Author: David Glick
Publisher: John Wiley & Sons
Biochemical analysis is a rapidly expanding field and is a key component of modern drug discovery and research. Methods of Biochemical Analysis provides a periodic and authoritative review of the latest achievements in biochemical analysis. Founded in 1954 by Professor David Glick, Methods of Biochemical Analysis provides a timely review of the latest developments in the field.