The Method of Enzyme ActionThe Method of Enzyme Action



Author: Beatty James 1870-

Publisher: Hardpress Publishing

ISBN: 1313303941

Category:

Page: 164

View: 479

Unlike some other reproductions of classic texts (1) We have not used OCR(Optical Character Recognition), as this leads to bad quality books with introduced typos. (2) In books where there are images such as portraits, maps, sketches etc We have endeavoured to keep the quality of these images, so they represent accurately the original artefact. Although occasionally there may be certain imperfections with these old texts, we feel they deserve to be made available for future generations to enjoy.

The Method of Enzyme Action Classic Reprint The Method of Enzyme Action Classic Reprint



About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work.

Author: James Beatty

Publisher: Forgotten Books

ISBN: 0267983433

Category:

Page: 178

View: 450

Excerpt from The Method of Enzyme Action In offering this working hypothesis for consideration I do not claim that the central idea is in any respect new. All I can say is that I have not met anywhere with the conception of the essential unity of the action of both hydrolysing and oxidising enzymes, nor an account of the mechanism similar to that given. It is quite possible, of course, that both have been previously suggested. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.

Kinetics of Enzyme ActionKinetics of Enzyme Action



A particularly useful variant on standard HPLC methods of measuring enzyme activity is the so-called “semi-continuous” method of HPLC analysis (Harrison et al. 1989). According to this method, the reaction solution of enzyme and ...

Author: Ross L. Stein

Publisher: John Wiley & Sons

ISBN: 1118084403

Category:

Page: 320

View: 600

Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the pharmaceutical industry has in this field. Beginning with the most basic principles pertaining to simple, one-substrate enzyme reactions and their inhibitors, and progressing to a thorough treatment of two-substrate enzymes, Kinetics of Enzyme Action: Essential Principles for Drug Hunters provides biochemists, medicinal chemists, and pharmaceutical scientists with numerous case study examples to outline the tools and techniques necessary to perform, understand, and interpret detailed kinetic studies for drug discovery.

Methods to Determine Enzymatic ActivityMethods to Determine Enzymatic Activity



The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text.

Author: Alane Beatriz Vermelho

Publisher: Bentham Science Publishers

ISBN: 9781608053001

Category:

Page: 332

View: 792

Methods to Determine Enzymatic Activity is a textbook about industrial enzymes. The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text. The main objective of this textbook is to provide readers information focused on the current analysis methods of enzymatic activity at qualitative and quantitative levels. Each chapter is about one specific enzyme and contains information about its substrate and some biochemical properties. The methodologies are presented as an experimental protocol allowing interested readers to reproduce the experimental methods detailed within the textbook. These protocols contain the principle of the technique, materials, methods, and all steps necessary for the determination of enzyme activity and interpretation of results. Each methodology is illustrated with photos and schemes for a better and clear understanding. This book, therefore, uniquely brings modern analysis techniques of industrial enzymes in a single easy to understand volume. This textbook is suitable for undergraduate enzymology courses and advanced industrial biotechnology and microbiology courses.

Enzyme NutritionEnzyme Nutrition



In the year 1958 , I devised a method of capturing enzyme activity on film by motion picture microphotography . The description and plates shown in Figure 2.1 are reproduced by permission of the National Enzyme Company . The action ...

Author: Edward Howell

Publisher: Penguin

ISBN: 0895292211

Category:

Page: 192

View: 197

Why is eating food in its natural state, unprocessed and unrefined, so vital to the maintenance of good health? What is lacking in our modern diet that makes us so susceptible to degenerative disease? What natural elements in food may play a key role in unlocking the secrets of life extension? These fascinating questions, and many more, are answered in Enzyme Nutrition. Written by one of America’s pioneering biochemists and nutrition researchers, Dr. Edward Howell, Enzyme Nutrition presents the most vital nutritional discovery since that of vitamins and minerals—food enzymes. Our digestive organs produce some enzymes internally, however food enzymes are necessary for optimal health and must come from uncooked foods such as fresh fruits and vegetables, raw sprouted grains, unpasteurized dairy products, and food enzyme supplements. Enzyme Nutrition represents more than fifty years of research and experimentation by Dr. Howell. He shows us how to conserve our enzymes and maintain internal balance. As the body regains its strength and vigor, its capacity to maintain its normal weight, fight disease, and heal itself is enhanced.

Methods of Soil EnzymologyMethods of Soil Enzymology



Methods in Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes.

Author: Richard P. Dick

Publisher: ASA-CSSA-SSSA

ISBN: 0891188541

Category:

Page: 395

View: 502

Methods in Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background information, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.

Automated Enzyme AssaysAutomated Enzyme Assays



In the face of rapid developments in automated techniques, this book presents an excellent guide to present trends.

Author: D.B. Roodyn

Publisher: Elsevier

ISBN: 9780080887739

Category:

Page: 224

View: 172

In the face of rapid developments in automated techniques, this book presents an excellent guide to present trends. It provides information on: principles and terminology of enzyme automation; automatic methods illustrated by the Technicon system; semi-automatic methods; interrupted-flow and discrete-sampling systems; single-enzyme analysis; multiple-enzyme analysis (M.E.A.); enzyme characterization; calculation of enzyme activities from instrument readings; generalized systems for enzyme automation; Appendices I. Published automated enzyme assays, II. Terminology used in enzyme automation, III. Apparatus used in enzyme automation, IV. Computer program for generalized enzyme automated system.

Clinical and Experimental Toxicology of Organophosphates and CarbamatesClinical and Experimental Toxicology of Organophosphates and Carbamates



The method of Potter [92] also has relatively low contamination levels of 0.2–0.4%. ... George and Abernethy [44] have made use of propionylthiocholine as substrate and the detergent benzethonium chloride to stop the enzyme action.

Author: Bryan Ballantyne

Publisher: Elsevier

ISBN: 9781483193656

Category:

Page: 662

View: 591

Clinical & Experimental Toxicology of Organophosphates and Carbamates considers the histochemical localization of cholinesterase in anticholinesterase poisoning. This book is organized into eight parts encompassing 59 chapters that discuss the pharmacology and toxicology of carbamates, as well as the neurobehavioral toxicology of anticholinesterases. Some of the topics covered in the book are the overview of biological and clinical aspects of organophosphates and carbamates; biochemical determination of cholinesterase activity in fluids and tissues; intermediate syndrome in anticholinesterase neurotoxicity; structure-activity relationships and anticholinesterase activity; and the molecular events in delayed neuropathy. Other parts deal with the cardiac effects of anticholinesterase agents and the ecotoxicological consequences of interactions between avian esterases and organophosphorus compounds. These topics are followed by discussions on the analysis of the cutaneous and respiratory tract absorption of anticholinesterases and the use of organophosphorus compounds as chemical warfare agents. The final parts are devoted to the toxicity of anticholinesterases to aquatic organisms. The book can provide useful information to toxicologists, doctors, students, and researchers.

Handbook of Detection of Enzymes on Electrophoretic GelsHandbook of Detection of Enzymes on Electrophoretic Gels



Examples of this are the detection of trypsin (3.4.21.4 — T; Other Methods, A), esteroprotease (3.4.21.40 — EP, Method 2), and carbonic anhydrase (4.2.1.1 — CA, Method 1). The orthophosphate, liberated by enzymatic activity, ...

Author: Gennady P. Manchenko

Publisher: CRC Press

ISBN: 9781420040531

Category:

Page: 568

View: 615

Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish

Advances in Applied MicrobiologyAdvances in Applied Microbiology



al., 1931) formed due to enzyme action. Most of the old methods were based on the titration of the gallic acid liberated by enzyme action (Freudenberg et al., 1927; Nishira, 1961; Haslam and Stangroom, 1966) and hence do not yield ...

Author:

Publisher: Academic Press

ISBN: 0080564585

Category:

Page: 358

View: 556

Praise for the Series "No laboratory scientist, field worker or technical administrator can afford to pass it up." --ASM News "The topics are well supported by an extensive bibliography and provide a rich source of current information." --Biopharm

Methods of Biochemical AnalysisMethods of Biochemical Analysis



authors, a further disadvantage is that it cannot be used with crude enzyme preparations of low specific activity, because the contribution of the change in conductivity due to enzyme action may be very small, as compared with that of ...

Author: David Glick

Publisher: John Wiley & Sons

ISBN: 9780470110843

Category:

Page: 567

View: 908

Biochemical analysis is a rapidly expanding field and is a key component of modern drug discovery and research. Methods of Biochemical Analysis provides a periodic and authoritative review of the latest achievements in biochemical analysis. Founded in 1954 by Professor David Glick, Methods of Biochemical Analysis provides a timely review of the latest developments in the field.