Circulating Nucleic Acids in Plasma and SerumCirculating Nucleic Acids in Plasma and Serum



This volume encompasses the proceedings of the 6th international conference on circulating nucleic acids in plasma and serum held from the 9th to the 11th of November 2009 in Hong Kong.

Author: Peter B. Gahan

Publisher: Springer Science & Business Media

ISBN: 9048193826

Category:

Page: 285

View: 345

DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.

Circulating Nucleic Acids in Plasma Or SerumCirculating Nucleic Acids in Plasma Or Serum



An Overview on the Isolation and Analysis of Circulating Tumor DNA in Plasma
and Serum JIN JEN , LI WU , AND DAVID SIDRANSKY Division of Head and
Neck Cancer Research , Departments of Otolaryngology and Oncology , The
Johns ...

Author: Philippe Anker

Publisher:

ISBN: 157331269X

Category:

Page: 188

View: 217

Free DNA present in both healthy and diseased human plasma has been found to express specific point mutations that may serve as diagnostic signposts - perhaps to reliable, non-invasive tests for breast and lung cancer, haematopoetic malignancies, colorectal or pancreatic carcinoma, and other tumours. In another research area, foetal DNA has been detected in maternal serum which presents an early-horizon alternative in utero test for gender. A parallel discovery of rearranged immunoglobin heavy-chain DNA in the plasma of patients wit Hodgkin's disease or acute B cell leukaemia has also emerged. These fast-emerging indicators suggest that it is time to standardize research techniques and develop protocols that can take a measure of circulating DNA's usefulness as a diagnostic tool. This volume, taken from the proceedings of a conference in August 1999, discusses such issues.

Circulating Nucleic Acids in Serum and Plasma CNAPS IXCirculating Nucleic Acids in Serum and Plasma CNAPS IX



The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids.

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9783319420448

Category:

Page: 220

View: 144

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders“ between them.
Numerous questions remain regarding the origin, release, clearance, clinical utility, and possible functionality of circulating nucleic acids. Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.

Circulating Nucleic Acids in Plasma and Serum VCirculating Nucleic Acids in Plasma and Serum V



This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter ...

Author: Peter B. Gahan

Publisher: Blackwell Pub

ISBN: STANFORD:36105132368627

Category:

Page: 345

View: 244

DNA and RNA fractions have been isolated from whole blood, serum, plasma, the surface of blood cells, and urine from both healthy persons and patients. Specific fragments have been identified as being related to particular disorders, for example, diabetes, cancer, myocardial infarction, and stroke. The ability to isolate these fractions and assess their relationship to specific diseases makes early noninvasive diagnosis possible. This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter cells and exhibit biological activity, isolating and testing fetal cells from maternal blood, and discussing the clinical implications of these discoveries and developing the techniques that will bring them into clinical application. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member

Circulating Nucleic Acids in Plasma serum III and Serum ProteomicsCirculating Nucleic Acids in Plasma serum III and Serum Proteomics



Quantification of Circulating DNA in the Plasma and Serum of Cancer Patients
BRET TABACK , “ STEVEN J. O'DAY , AND DAVE S.B. HOON " Department of
Molecular Oncology and bCancer Institute Medical Group , John Wayne Cancer ...

Author: Dave S. B. Hoon

Publisher:

ISBN: UVA:X004817516

Category:

Page: 325

View: 108

Numerous questions remain regarding the origin, release, clearance, clinical utility, and possible functionality of circulating nucleic acids. Serum proteomics is introduced as a new topic to CNAPS and is expected to provide yet another platform for breakthrough discoveries. Serum proteomics topics will address both cancer and early pregnancy.

Circulating Nucleic Acids in Plasma and Serum VCirculating Nucleic Acids in Plasma and Serum V



48 According to Bianchi et al . , 47 the ma - significantly higher circulating plasma
DNA jority of fetal DNA during pregnancy originates concentrations than in
normal controls , and from the placenta . Free DNA in the fetus may even higher ...

Author: Peter B. Gahan

Publisher: Blackwell Pub

ISBN: STANFORD:36105132368627

Category:

Page: 345

View: 137

DNA and RNA fractions have been isolated from whole blood, serum, plasma, the surface of blood cells, and urine from both healthy persons and patients. Specific fragments have been identified as being related to particular disorders, for example, diabetes, cancer, myocardial infarction, and stroke. The ability to isolate these fractions and assess their relationship to specific diseases makes early noninvasive diagnosis possible. This volume reports on the most recent progress in this field, including chapters on developing protocols for nucleic acid extraction, determining the origin of the circulating nucleic acids, the capacity of these nucleic acids to enter cells and exhibit biological activity, isolating and testing fetal cells from maternal blood, and discussing the clinical implications of these discoveries and developing the techniques that will bring them into clinical application. NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member

Circulating Nucleic Acids in Serum and Plasma u2013 CNAPS IXCirculating Nucleic Acids in Serum and Plasma u2013 CNAPS IX



The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids.

Author: Peter B. Gahan

Publisher:

ISBN: OCLC:1066501894

Category:

Page: 220

View: 790

The book will present the progress made since the last meeting in fall 2013 concerning the analysis of circulating extra-cellular nucleic acids. There are a modest number of laboratories involved in this field, nevertheless the number of papers published by researchers is extensive. The articles which will be published in this meeting report will be a valuable contribution for researchers and research students alike and will help them to stay on top of the developments in different research areas and to „cross borders? between them.

Circulating Nucleic Acids in Early Diagnosis Prognosis and Treatment MonitoringCirculating Nucleic Acids in Early Diagnosis Prognosis and Treatment Monitoring



FEBS Lett 581:795–799 Tsang JC, Lo YM (2007) Circulating nucleic acids in
plasma/serum. Pathology 39:197–207 Wagner J (2012) Free DNA – new
potential analyte in clinical laboratory diagnostics? Biochem Med (Zagreb) 22:24
–38 El ...

Author: Peter B. Gahan

Publisher: Springer

ISBN: 9789401791687

Category:

Page: 471

View: 746

DNA and RNA fractions can be isolated from a variety of body fluids including whole blood, serum, plasma, urine, saliva and cerebrospinal fluid from both patients and healthy individuals. Such isolates can be exploited in the early detection of clinical disorders, stratification of patients for treatment, treatment monitoring and clinical follow-up. In addition, the use in fetal medicine allows the early detection of fetal sex, Rh factor and aneuploid disorders as well as following both fetal and premature born infant development. This volume is intended as a primer for those who are interested in entering the field of circulating nucleic acids. The areas covered in this volume include: · Background and general biology of circulating nucleic acids · Methodology · Applications of circulating nucleic acids · Quality Assurance · Ethics

Handbook of Nucleic Acid PurificationHandbook of Nucleic Acid Purification



16.1.1 CELL-FREE DNA IN PLASMA AND SERUM The presence of circulating
nucleic acids in blood plasma was first described by Mandel and Métais in 1947 [
1], who demonstrated that extracellular nucleic acids could be detected in the ...

Author: Dongyou Liu

Publisher: CRC Press

ISBN: 9781420070972

Category:

Page: 584

View: 218

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic acids, and these have become a cornerstone for all branches of modern biological sciences. The Handbook of Nucleic Acid Purification provides researchers and students with an all-encompassing volume on nucleic acid extraction strategies. Due to the complexities within prokaryotic and eukaryotic cells, purification of the nucleic acids often forms a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. Bringing together contributions from leading researchers, the handbook presents a comprehensive catalog of nucleic acid isolation methods. It includes dedicated sections on strategies for viruses, bacteria, fungi, parasites, insects, mammals, and plants, as well as for ancient samples, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter in this handbook: Explores the biological background important to understanding specific organisms and specimens Reviews principles and current techniques for efficient isolation Discusses challenges and future trends relating to improved recovery of nucleic acids Besides providing an updated, reliable reference for anyone with an interest in molecular biology, this book offers a practical guide for clinical, forensic, and research scientists involved in molecular analysis of biological specimens. It also constitutes a convenient resource for students in other areas of biological sciences, and an indispensable roadmap for both new and experienced researchers wishing to acquire or sharpen their skills in nucleic acid preparation.

Cumulated Index MedicusCumulated Index Medicus



Mattagajasingh SN , et al . from tumor cells and from plasma DNA of patients **
drugs that inhibit nucleoside transport , induce DNA Mol Cell Biochem ... of
plasma / serum DNA of care In vitro DNA synthesis in freshly separated human
breast The molecular perspective : DNA . ... 2000 ; 5 ( 1 ) : 81-2 Clinical
significance of circulating DNA microscit assay : relationship to the long - term
outcome of patients .

Author:

Publisher:

ISBN: UIUC:30112042535085

Category:

Page:

View: 252

Anticancer ResearchAnticancer Research



To the best of our knowledge , this was also the first study to investigate the
relationship between the plasma expression of circulating hТERT DNA and the
serum level of CA 15 . 3 in BC patients before surgery , and to analyze their
association ...

Author:

Publisher:

ISBN: NWU:35558005724337

Category:

Page:

View: 453

Journal of the National Cancer InstituteJournal of the National Cancer Institute



use of different procedures to quantify circulating DNA ) on the variability of
quantitative assays . ... REFERENCES NOTES ( 1 ) Anker P , Mulcahy H , Stroun
M. Circulating nucleic acids in plasma and serum as a noninvasive investigation
for ...

Author:

Publisher:

ISBN: NWU:35558005263468

Category:

Page:

View: 140

"Summaries of papers" contained in the journal accompany each issue, 19--

Extracellular Nucleic AcidsExtracellular Nucleic Acids



This book covers nearly all of the newly developing fields related to extracellular nucleic acids, including those of basic biology, ecology and the medical sciences, and provides readers with the latest knowledge on them.

Author: Yo Kikuchi

Publisher: Springer Science & Business Media

ISBN: 3642126170

Category:

Page: 232

View: 743

Extracellular nucleic acids have recently emerged as important players in the fields of biology and the medical sciences. In the last several years, extracellular nucleic acids have been shown to be involved in not only microbial evolution as genetic elements but also to have structural roles in bacterial communities, such as biofilms. Circulating DNA and RNA have been found in human blood and expected to be useful as non-invasive markers for the diagnosis of several diseases. In addition, extracellular nucleic acids have attracted attention as active modulators of the immune system of higher organisms, including humans. This book covers nearly all of the newly developing fields related to extracellular nucleic acids, including those of basic biology, ecology and the medical sciences, and provides readers with the latest knowledge on them.